Port Byron preserves a broad cross-section of its railroad, canal, commercial, civic, industrial, and religious past through sites including the Park Hotel, built by Samuel Lamkin in 1835 and known during the busy railroad era as the National Hotel; the Masonic Block, built just before the Civil War with shops below, meeting rooms above, a third-floor hall used for dances and theater, and a village board meeting held there in 1851; the trolley station later used by the American Legion, tied to the Rochester, Syracuse and Eastern Railroad from 1908 to 1931; the site of the first post office; churches including the Federated Church, built in 1843 as a Presbyterian church and repaired after an 1887 fire, Saint John's Church, whose congregation had met since 1858 before buying land in the late 1890s, and the Baptist Church, built about 1834 and later owned by the Masons. The village also includes the Brick House built by A.W. Green, a former Cayuga County sheriff; the birthplace house of opera star Richard Bonelli, also known as George Bunn, born on February 6, 1884; Wilt's Mill, first owned by John Tanner and later bought by Delvin Wilt Sr. around 1917; the Erie House, a tavern built by Peter Van Detto and regarded as one of the few surviving Erie Canal business buildings; the remains of Lock 52, a double lock built in 1851 to raise and lower boats between different water levels; a 1907 power house serving the railroad; King's Tavern, a pre-Erie Canal halfway stop between Buffalo and Albany; the Henry Wells home, where Wells lived as a shoemaker before delivering canal packages and later forming Well-Fargo Express with a friend from Weedsport; a feeder line for the Erie Canal; the original 1890 firehouse; Warren's Mill, the fourth mill on its site, built in 1877 and later run by Irving Warren and his son Richard; the mill pond and dam that powered mills through a raceway and flume; Doctor Stewart's House, later used for high school classes after the Port Byron school burned in December 1935; the site of the Eagle Hotel Inn, where Brigham Young married his first wife on October 24, 1824, before the inn burned in the winter of 1856; Brigham Young's former home; the accessible stretch of enlarged Erie Canal and Conservation Trail opened to the public in 1987; and the Tanner-Shelter Drydock, one of the busiest on the Erie Canal from the 1870s until 1917.