The Milwaukee and Mississippi Railroad Company, immediate successor of the Milwaukee and Waukesha formed in 1850, built the first railroad line across Wisconsin between Milwaukee and Prairie du Chien. Edward H. Brodhead was hired as Chief Engineer in 1851 and selected Mazomanie as a site for fueling, watering, and servicing locomotives. The railroad was officially opened to Mazomanie on June 7, 1856. During the 1800s, two special trains operated from the village: a wood train that obtained wood to fuel the steam locomotives, and a construction train whose crew performed emergency work both east and west of Mazomanie. Quarters on the second floor of the depot provided housing and dining facilities for these and other railroad crews. At the height of nineteenth-century railroading, the Mazomanie railroad corridor had as many as five side tracks in addition to the main line, and an engine house, turntable, and water tower stood between Brodhead and Cramer Streets. A branch line to Sauk City and Prairie du Sac was completed in 1881, and a wye was added in 1942. On July 1, 1982, the Wisconsin Department of Transportation bought the right-of-way, and on July 6, 1982, the last train operated by the Milwaukee Road passed through the village. Later operators included the Wisconsin and Western, the Wisconsin and Calumet, and the Wisconsin and Southern Railroads, and in 1992 the track and roadbed were rehabilitated by the Wisconsin River Rail Transit Commission.