Amphion began with the establishment of Atascosa County's first courthouse, believed to have been built near this site at the county seat of Navatasco in 1857. Located within the seventeen thousand-acre ranch of Jose Antonio Navarro, a prominent local rancher and signer of the Texas Declaration of Independence, Amphion was at one time a thriving community with several general stores, a hotel, post office, blacksmith shop, cotton gin, tannery, churches, fraternal lodges, and a school. Amphion Cemetery was established about 1870 on land donated by Roy Jenkins and Frank Lozano. Although the earliest recorded gravesite is that of Laura Underwood, who died in 1891, one gravestone bears the year 1800, which local tradition claims marks the grave of a young boy killed by Indians. The cemetery contains the graves of at least two veterans of the American Civil War. After railroad lines were built through Atascosa County in 1907 and 1927 along routes that bypassed Amphion, business activity declined and the community eventually dissolved, leaving virtually only the cemetery as the remains of the former town.