Jonesville is an unincorporated community in Alachua County, Florida, United States. It is located roughly at the intersection of County Road 241 and State Road 26 (Newberry Road). Jonesville has no city government but is an informal name for a region on Newberry Road between Gainesville and Newberry. While no clear delineation has been made for what includes Jonesville, its eastern boundary is 122nd street (colloquially known as Parker Road), due to the City of Gainesville owning up to the eastern side of the road. All the other limits are vague. Jonesville may extend as far west as to 170th St. Approximate northern and southern markers for the region are NW 39th Avenue and SW 8th Avenue, respectively.

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